Cell Culture Range

DNA/RNA Isolation Kits

NucleoBond RNA/DNA 80 and 400 kits are designed for the isolation and purification of up to 80 or 400 µg of ultra-pure total RNA, respectively, plus a separate genomic DNA fraction. A special manufacturing process guarantees RNase-free handling. These kits contain NucleoBond AXR columns (anion-exchange columns for the highest purity and yield) and all of the required buffers. Due to the resolving power of NucleoBond anion-exchange resin, contaminants are washed away, and RNA and genomic DNA can be eluted separately.

Real Time PCR

The AAVpro Titration Kit (for Real Time PCR) is a kit for determining the titer of adeno-associated virus (AAV) using real-time PCR. This kit contains all of the reagents necessary for extraction of AAV particles from AAV vector-producing cells and real-time PCR assay of virus genome. This kit allows more precise and rapid quantification than conventional DNA blotting or ELISA methods. Quantification is based on amplification of the ITRs (inverted terminal repeats) of AAV, and therefore titer measurement is possible for various serotypes of AAV.

Next Generation Sequencing

Our broad NGS portfolio provides unmatched sensitivity and reproducibility for all of your demanding sequencing applications—regardless of sample type or input amount.

mRNA & cDNA Synthesis

Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription using an optimized, highly efficient T7 RNA polymerase. The kit includes separate NTPs for easy optimization or replacement with modified nucleosides, such as pseudouridine. The kit synthesizes up to 200 μg or more of mRNA per 20 μl reaction, includes DNase I to digest the template DNA after the in vitro transcription reaction and a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications.
cDNA cloning is used for structural analysis of genes and RNAs, and expression of target proteins. Generating a cDNA library involves synthesizing double-stranded cDNA that is complementary to the target mRNA, inserting the cDNA into a vector of bacterial or viral origin, and introducing the vector into bacterial or eukaryotic cells. The cloned cDNA can be then used for analysis, and in vitro transcription and translation. The PrimeScript Double Strand cDNA Synthesis Kit synthesizes double-stranded cDNA from polyA+ RNA of animal or plant origin using the Gubler-Hoffman method.

Cloning

We offer a complete line of best-in-class reagents to fit every cloning project. With streamlined methods and high-performance reagents, you can easily move forward in your research. Utilize the power of In-Fusion Cloning kits to perform ligation-independent cloning of PCR products into any vector, at any site of linearization. We also offer traditional ligation-based kits, in addition to a variety of restriction enzymes and modifying enzymes.

Protein Research

• Purification products
• Two-hybrid and one-hybrid systems
• Mass spectrometry reagents
• Expression vectors & systems
• Antibodies and immunoprecipitation
• Accessory enzymes

RNA Interference Products

RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules. RNAi can be used for identification and validation of drug targets as well as in the discovery of cell-signaling pathways that are vital to organism survival and disease control. This technology is crucial to the development of new therapeutic drugs and treatments.

Human Pathogen Detection

• Bacteria & Yeast
• Fungi & Protozoa
• Parasite
• Virus

Plant Pathogen Detection

• Bacteria, Fungi & Yeast
• Viroids & Viruses

Mycoplasma Detection Kits

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

Microbiology Kits and Assays

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Norgen offers sensitive PCR-based detection kits to allow for the detection and diagnosis of mycoplasma contamination in cell cultures.

NGS Library Preparation Kits

• Small RNA Library Preparation Kits
• 16s rRNA Library Preparation Kits
• ITS Library Preparation Kits